Cycloheximide

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
2g	$180	Check With Us
5g	$300	Check With Us
10g	$450	Check With Us

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Cat #: V3042 CAS #: 66-81-9 Purity ≥ 98%

Description: Cycloheximide, produced by S. griseus, is a glutarimide antibiotic and an inhibitor of protein synthesis in eukaryotes (but not prokaryotes) with IC50 of 5-50 μM (IC50s of 532.5 nM and 2880 nM for protein synthesis and RNA synthesis in vivo, respectively).

References: Schneider-Poetsch T, et al. Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nat Chem Biol. 2010 Mar;6(3):209-217.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	281.35
Molecular Formula	C15H23NO4
CAS No.	66-81-9
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: >14.1mg/mLr
	Water: <1mg/mLr
	Ethanol:<1mg/mL
Synonyms	Naramycin A; Actidione; Cycloheximide; NSC 185

Protocol	In Vitro	Cycloheximide (CHX) is the most common laboratory reagent used to inhibit protein synthesis. Cycloheximide has been shown to block the elongation phase of eukaryotic translation. Cycloheximide binds the ribosome and inhibits eEF2-mediated translocation. Surprisingly, Cycloheximide allows one complete translocation cycle to proceed before halting any further elongation. Cycloheximide has been speculated that Cycloheximide requires an E-site bound deacylated tRNA for activity

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.5543 mL	17.7715 mL	35.5429 mL	71.0858 mL
5mM	0.7109 mL	3.5543 mL	7.1086 mL	14.2172 mL
10mM	0.3554 mL	1.7771 mL	3.5543 mL	7.1086 mL
20mM	0.1777 mL	0.8886 mL	1.7771 mL	3.5543 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.