Bendamustine HCl (SDX-105)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
1g	$400	Check With Us
5g	$1250	Check With Us
10g	$1875	Check With Us

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Cat #: V1466 CAS #: 3543-75-7 Purity ≥ 98%

Description: Bendamustine HCl (formerly also called SDX-105; EP-3101; Cytostasane; DD6304600; Treanda), the hydrochloride salt of bendamustine which is a bifunctional mechlorethamine derivative, is a potent DNA-alkylating/ cross-linking / damaging agent (IC50 = 50 μM in cell-free assay) approved for cancer treatment.

References: Ackler S, et al. Navitoclax (ABT-263) and bendamustine ± rituximab induce enhanced killing of non-Hodgkin's lymphoma tumours in vivo. Br J Pharmacol. 2012 Oct;167(4):881-91.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	394.72
Molecular Formula	C16H21Cl2N3O2.HCl
CAS No.	3543-75-7
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 78 mg/mL (197.6 mM)r
	Water: 2 mg/mL (5.0 mM)r
	Ethanol: 17 mg/mL (43.1 mM)
Solubility In Vivo	1% DMSO+30% polyethylene glycol+1% Tween 80: 30 mg/mL
Synonyms	SDX-105 (Cytostasane) HCl; EP-3101; SDX105; EP 3101; SDX 105; SDX-105; EP3101; DD6304600; Bendamustinum; Bendamustina; Ribomustin. Brand name: Treanda.

Protocol	In Vivo	Bendamustine (25 mg/kg, i.v.) shows potent inhibition on the growth of tumor cells by 91%, 99% and 95% for DoHH-2, Granta 519 and RAMOS models, respectively. Moreover, the antitumor effect of Bendamustine is enhanced by rituximab in DoHH-2 and RAMOS models, but not in Granta 519 model

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.5334 mL	12.6672 mL	25.3344 mL	50.6688 mL
5mM	0.5067 mL	2.5334 mL	5.0669 mL	10.1338 mL
10mM	0.2533 mL	1.2667 mL	2.5334 mL	5.0669 mL
20mM	0.1267 mL	0.6334 mL	1.2667 mL	2.5334 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.