Hematoxylin (Haematoxylin)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
50g	$250	Check With Us
100g	$450	Check With Us
200g	$375	Check With Us

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Cat #: V2021 CAS #: 517-28-2 Purity ≥ 98%

Description: Hematoxylin (also known as Hydroxybrazilin; Natural Black 1) is a natural compound that forms strongly colored complexes with certain metal ions, notably Fe(III) and Al(III) salts and a kind of stain in histology.

References: M Titford. The long history of hematoxylin. Biotech Histochem. Mar-Apr 2005;80(2):73-8.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	302.28
Molecular Formula	C16H14O6
CAS No.	517-28-2
Storage	-20℃ for 3 years in powder formrr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 61 mg/mL (201.8 mM)rr
	Water: 61 mg/mL (201.8 mM)rr
	Ethanol: 61 mg/mL (201.8 mM)
SMILES Code	O=C1C=C2CC3(O)C(C4=CC=C(O)C(O)=C4OC3)=C2C=C1O
Synonyms	Haematoxylin; Natural Black 1; Hematein, haematein, Hematoxylin, Haematoxylin, AI3-63013, AI3 63013, AI363013, NSC 7333, NSC-7333, NSC7333

Protocol	In Vitro	When exposed to air, Hematoxylin is oxidized to reddish brown hematein. When oxidized to its hematein form and combined with a mordant, usually a metal salt, Hematoxylin stains tissue sections a deep blue to black color depending on the staining method. By itself, Hematoxylin is also amphoteric in its hematein form; it is red at acid pH and blue at alkaline pH. Differentiation following Hematoxylin staining removes nonspecific staining

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.3082 mL	16.5410 mL	33.0819 mL	66.1638 mL
5mM	0.6616 mL	3.3082 mL	6.6164 mL	13.2328 mL
10mM	0.3308 mL	1.6541 mL	3.3082 mL	6.6164 mL
20mM	0.1654 mL	0.8270 mL	1.6541 mL	3.3082 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.