NAE-IN-M22
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $1200 | Check With Us |
| 250mg | $1850 | Check With Us |
| 500mg | $2775 | Check With Us |
: Cat #: V2276 CAS #: 864420-54-2 Purity ≥ 99%
Description: NAE-IN-M22 is a selective and reversible inhibitor of NEDD8 activating enzyme.
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- Physicochemical and Storage Information
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- Related Biological Data
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- Quality Control Documentation
| Molecular Weight (MW) | 363.32 |
|---|---|
| Molecular Formula | C20H24Cl2N2 |
| CAS No. | 864420-54-2 |
| SMILES Code | ClC1=CC=C(C(Cl)=C1)CCNC2CCN(CC3=CC=CC=C3)CC2 |
| Synonyms | M22; M-22; M 22; M22 NEDD8 inhibitor; |
| Protocol | In Vitro | M22 (0.37-90 μM; 24 h) blocks neddylation pathway selectively and suppresses degradation of CRL substrates in A549 cells. M22 (15-30 μM; 36 h) promotes apoptosis in A549 cell line. M22 (0.1-100 μM; 48 h) inhibits A549 cell proliferation completely at 30 µM (GI50=5.5 µM, GI90=19.3 µM). |
|---|---|---|
| In Vivo | M22 (0.36-90 μM) has low acute toxicity in zebrafish model. | |
| Animal model | M22 (60 mg/kg; i.p. once daily for 14 d) inhibits tumor growth in nude mice bearing AGS xenografts. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.7524 mL | 13.7620 mL | 27.5239 mL | 55.0479 mL |
| 5mM | 0.5505 mL | 2.7524 mL | 5.5048 mL | 11.0096 mL |
| 10mM | 0.2752 mL | 1.3762 mL | 2.7524 mL | 5.5048 mL |
| 20mM | 0.1376 mL | 0.6881 mL | 1.3762 mL | 2.7524 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.