BTZO-1 | CAS 99420-15-2

BTZO-1

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
100mg	$675	Check With Us
200mg	$1010	Check With Us
500mg	$1705	Check With Us

: [email protected] [email protected]

Cat #: V17181 CAS #: 99420-15-2 Purity ≥ 98%

Description: BTZO-1, a cardioprotective compound, is a novel and potent activator of the glutathione S-transferase Ya subunit (GST Ya) gene-antioxidant response element (ARE) by interacting with MIF, it binds to Macrophage migration inhibitory factor (MIF) with a Kd value of 68.6 nM, and its binding requires the N-terminal Pro1.

References: Kimura H, et al. BTZO-1, a cardioprotective agent, reveals that macrophage migration inhibitory factor regulates ARE-mediated gene expression.Chem Biol. 2010 Dec 22;17(12):1282-94.

Top Publications Citing Invivochem Products

Product Catalog 2022

Guide to Product Handling

Publications Citing InvivoChem Products

Product Promise

Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	240.28
Molecular Formula	C13H8N2OS
CAS No.	99420-15-2
SMILES Code	O=C1N=C(C2=NC=CC=C2)SC3=CC=CC=C13
Synonyms	BTZO1; BTZO 1; BTZO-1

Protocol	In Vitro	BTZO-1 (0.37 μM; 21 hours) upregulates mRNA protein levels of GST Ya and HO-1 in cardiomyocytes. BTZO-1 (0-330 nM; 24 hours) dose-dependently suppressed serum deprivation-induced cardiomyocyte death; the minimum effective concentration (MEC1.5), the concentration of a sample necessary to give a 50% increase in cell viability, is 16 nM.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	4.1618 mL	20.8091 mL	41.6181 mL	83.2362 mL
5mM	0.8324 mL	4.1618 mL	8.3236 mL	16.6472 mL
10mM	0.4162 mL	2.0809 mL	4.1618 mL	8.3236 mL
20mM	0.2081 mL	1.0405 mL	2.0809 mL	4.1618 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.