SPD-304 | CAS 869998-49-2

SPD-304

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
100mg	$2325	To Be Confirmed
200mg	$3485	To Be Confirmed
500mg	$5885	To Be Confirmed

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Cat #: V15104 CAS #: 869998-49-2 Purity ≥ 99%

Description: SPD-304 is a novel, potent and selective inhibitor of tumor necrosis factor α (TNFα) with an IC50 of 22 µM for preventing binding to the TNF Receptor 1 (TNFR1).

References: Molly M. He, et al. Small-Molecule Inhibition of TNF-α. Science 11 Nov 2005.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	547.61
Molecular Formula	C32H32F3N3O2
CAS No.	869998-49-2
SMILES Code	O=C1C(CN(C)CCN(C)CC2=CN(C3=CC=CC(C(F)(F)F)=C3)C4=C2C=CC=C4)=COC5=CC(C)=C(C)C=C15
Synonyms	SPD-304, SPD 304, SPD304

Protocol	In Vitro	SPD304 (2 μM) significantly rescues the survivability of aHSCs, reduces the production of lipid hydroxides, and increased intracellular GSH. The co-treatment of GA (75 μM) and SPD304 (2 μM), down-regulate TRADD almost 2-fold (w/o inhibitor vs. w/ inhibitor) and p−RIP3 1.4−fold compared to GA alone, and promotes caspase 8 activation.
Protocol	In Vivo	SPD304 cannot be used in vivo due to its high toxicity.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.8261 mL	9.1306 mL	18.2612 mL	36.5223 mL
5mM	0.3652 mL	1.8261 mL	3.6522 mL	7.3045 mL
10mM	0.1826 mL	0.9131 mL	1.8261 mL	3.6522 mL
20mM	0.0913 mL	0.4565 mL	0.9131 mL	1.8261 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.

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