(Rac)-Benpyrine

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
500mg	$1550	Check With Us
1g	$2150	Check With Us
5g	$5440	Check With Us

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Cat #: V40963 CAS #: 1333714-43-4 Purity ≥ 99%

Description: (Rac)-Benpyrine, the racemic mixture of Benpyrine, is a potent and orally bioactive TNF-α inhibitor with the potential for TNF-α mediated inflammatory and autoimmune disease research.

References: Hua Li, et al. Compound with TNF- α inhibitory activity and application. CN111320630A.

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Physicochemical and Storage Information
Protocol
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Stock Solution Preparation
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Molecular Weight (MW)	308.34
Molecular Formula	C16H16N6O
CAS No.	1333714-43-4
SMILES Code	O=C1N(CC2=CC=CC=C2)CC(NC3=C4N=CNC4=NC=N3)C1
Synonyms	Benpyrine racemate; (Rac)-Benpyrine;

Protocol	In Vitro	(Rac)-Benpyrine (Compound 001; 5-10 μM; 24 hours) treatment inhibits the expression level of TNF-α downstream pathway protein NF-κB in the nucleus in RAW264.7 macrophages.
Protocol	In Vivo	(Rac)-Benpyrine (Compound 001; 25-50 mg/kg; oral gavage; daily; for 3 weeks; Kunming mice) treatment obviously reduces the inflammatory cytokines IFN-γ, IL-1β and IL-6 in the blood serum, and the anti-inflammatory factor IL-10 is obviously increased, the foot swelling degree of the arthritis part is also obviously slowed down, and the weight index increase caused by the arthritis also has no obvious reduction.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.2432 mL	16.2159 mL	32.4317 mL	64.8635 mL
5mM	0.6486 mL	3.2432 mL	6.4863 mL	12.9727 mL
10mM	0.3243 mL	1.6216 mL	3.2432 mL	6.4863 mL
20mM	0.1622 mL	0.8108 mL	1.6216 mL	3.2432 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.