Pifithrin-α (PFTα) HBr
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $779 | Check With Us |
| 500mg | $1170 | Check With Us |
| 1g | $1755 | Check With Us |
: Cat #: V0014 CAS #: 63208-82-2 Purity ≥ 98%
Description: Pifithrin-α HBr ((PFTα hydrobromide; PFT-α) is a novel and potent inhibitor of p53, acting by inhibiting p53 protein transcription and p53-dependent transactivation of p53-responsive genes.
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| Molecular Weight (MW) | 367.3 |
|---|---|
| Molecular Formula | C16H18N2OS.HBr |
| CAS No. | 63208-82-2 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 67 mg/mL (182.41 mM)r |
| Water:<1 mg/mL (slightly soluble or insoluble)r | |
| Ethanol: 2 mg/mL (6.08 mM) | |
| Solubility In Vivo | 30% Propylene glycol, 5% Tween 80, 65% D5W: 30 mg/mL |
| SMILES Code | CC1=CC=C(C(CN2C(SC3=C2CCCC3)=N)=O)C=C1.[H]Br |
| Synonyms | PFT-alpha; Pifithrin-alpha; PFTalpha; Pifithrin alpha; PFT alpha |
| Protocol | In Vitro | Pifithrin-α (PFT-α) hydrobromideis a water-soluble compound that could suppress p53 protein transcription. Pifithrin-α can suppress glucose oxidase (GOX)-induced p53 protein increase in whole cell lysates, but cyclosporine A (CsA) fails to show such an inhibition effect. Notably, Pifithrin-α is able to block the GOX-induced Bcl-2 protein reduction. Similarly, it is Pifithrin-α rather than CsA that able to prevent the Bax increasing in whole cell lysates. |
|---|---|---|
| In Vivo | When the experiment is performed with Pifthirin-α (PFT-α) hydrobromide, a pharmacological p53 inhibitor, the percentage of annexin V-positive Foxe3-/- SMCs decreases to WT levels. Pifithrin-α (2.2 mg/kg, i.p.) significantly reduces the incidence of aortic rupture and intramural hematomas in Foxe3-/- mice that underwent transverse aortic constriction (TAC) (50% to 17%, P<0.05). After Pifthirin-α treatment, the mean diameter of the ascending aorta and the percentage of TUNEL-positive cells in the aortic media are also normalized to WT levels in surviving Foxe3-/- animals (P<0.05). |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.7226 mL | 13.6129 mL | 27.2257 mL | 54.4514 mL |
| 5mM | 0.5445 mL | 2.7226 mL | 5.4451 mL | 10.8903 mL |
| 10mM | 0.2723 mL | 1.3613 mL | 2.7226 mL | 5.4451 mL |
| 20mM | 0.1361 mL | 0.6806 mL | 1.3613 mL | 2.7226 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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The dilution calculator equation
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Volume(start)
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.