| Protocol | In Vitro | NSC59984 specifically restores p53 pathway signaling in mutant p53-expressing human colorectal cancer cells. NSC59984 induces cell death in tumor cells but not normal cells with little or no genotoxicity. NSC59984 induces mutant p53 protein degradation through MDM2-mediated ubiquitination in cancer cells. NSC59984 restores p53 pathway signaling through activation of p73. NSC59984 induces p73-dependent cell apoptosis in cancer. The EC50 of NSC59984 in most cancer cells is significantly lower than those of normal cells, with EC50 of 8.38 μM for p53-null HCT116 cells. |
|---|
| In Vivo | NSC59984 synergizes with CPT11 to induce cell death in mutant p53-expressing colorectal cancer cells and inhibits mutant p53-associated colon tumor xenograft growth in a p73-dependent manner. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
Preparing Stock Solutions
| Solvent volume to be added |
Mass (the weight of a compound) |
| Mother liquor concentration |
1mg | 5mg | 10mg | 20mg |
| 1mM | 3.7697 mL | 18.8487 mL | 37.6974 mL | 75.3949 mL |
|---|
| 5mM | 0.7539 mL | 3.7697 mL | 7.5395 mL | 15.0790 mL |
|---|
| 10mM | 0.3770 mL | 1.8849 mL | 3.7697 mL | 7.5395 mL |
|---|
| 20mM | 0.1885 mL | 0.9424 mL | 1.8849 mL | 3.7697 mL |
|---|
|
Quality Control Documentation
|
|
: 
