5-Hydroxymethyldeoxyuridine

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$1250	Check With Us
500mg	$1850	Check With Us
1g	$2775	Check With Us

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Cat #: V2215 CAS #: 5116-24-5 Purity ≥ 99%

Description: 5-Hydroxymethyldeoxyuridine is a nucleoside analog with potent anticancer and antiviral activities. It inhibits the replication of murine S180 lung carcinoma cells and Ehrlich ascites mammary carcinoma cells (ED50s = 8.5 and 4 μM, respectively) and multiple human leukemia cell lines (IC50s = 1.7-5.8 μM).

References: [1]. Shiau GT, et al. Synthesis and biological activities of 5-(hydroxymethyl, azidomethyl, or aminomethyl)-2'-deoxyuridine and related 5'-substituted analogues. J Med Chem. 1980 Feb;23(2):127-33.

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Molecular Weight (MW)	258.23
Molecular Formula	C10H14N2O6
CAS No.	5116-24-5
SMILES Code	OC[C@@H]1[C@H](C[C@H](N2C(NC(C(CO)=C2)=O)=O)O1)O
Synonyms	5-Hydroxymethyldeoxyuridine; α-Hydroxythymidine; 2'-Deoxy-5-(hydroxymethyl)uridine; 5-Hydroxymethyl-2'-deoxyuridine

Protocol	In Vitro	5-Hydroxymethyl-2'-deoxyuridine (0-10 μM) inhibits the replication of Sarcoma 180 cells and Ehrlich ascites carcinoma cells with ED50 values of 8.5 and 4 μM, respectively.
Protocol	In Vivo	5-Hydroxymethyl-2'-deoxyuridine (0, 5 and 50 mg/kg; i.p.; once) increases the survival of DBA/2 mice carrying L1210 leukemia.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.8725 mL	19.3626 mL	38.7252 mL	77.4503 mL
5mM	0.7745 mL	3.8725 mL	7.7450 mL	15.4901 mL
10mM	0.3873 mL	1.9363 mL	3.8725 mL	7.7450 mL
20mM	0.1936 mL	0.9681 mL	1.9363 mL	3.8725 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.