4-Hydroxyisoleucine

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
10mg	$80	To Be Confirmed

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Cat #: V29333 CAS #: 781658-23-9 Purity ≥ 99%

Description: 4-Hydroxyisoleucine (4-Hydroxy-L-isoleucine) is an amino acid (AA) extracted and purified from fenugreek seeds. 4-Hydroxyisoleucine (4-Hydroxy-L-isoleucine) has insulinotropic activity.

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Molecular Weight (MW)	147.1723
Molecular Formula	C6H13NO3
CAS No.	781658-23-9
Storage	Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	6.7948 mL	33.9738 mL	67.9476 mL	135.8951 mL
5mM	1.3590 mL	6.7948 mL	13.5895 mL	27.1790 mL
10mM	0.6795 mL	3.3974 mL	6.7948 mL	13.5895 mL
20mM	0.3397 mL	1.6987 mL	3.3974 mL	6.7948 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.