3-Oxocholic acid

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
1g	$550	Check With Us
2g	$900	Check With Us
5g	$1515	Check With Us

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Cat #: V39795 CAS #: 2304-89-4 Purity ≥ 99%

Description: 3-Oxocholic acid is an oxo-bile acid (secondary bile acid) and a metabolite of cholic acid isolated from C. perfringens, which is a bacteria found in the intestines of mammals and in the environment. 3-Oxocholic acid serum levels increase following ileal transposition surgery in rats.

References: Macdonald I, et al. Identification of 7alpha-, 12alpha-dihydroxy-3-oxo cholanoic acid as the major degradation product from cholic by C. perfringens. J Steroid Biochem. 1978 Apr;9(4):353-8.

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Molecular Weight (MW)	406.56
Molecular Formula	C24H38O5
CAS No.	2304-89-4
Synonyms	3-Oxocholic acid

Protocol	In Vivo	Male Goto-Kakizaki (GK) rats rats are subjected to Ileal transposition (IT) surgery. In the metabolomics analysis, compared with Sham-IT rats, 3-Oxocholic acid of IT rats is increased.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.4597 mL	12.2983 mL	24.5966 mL	49.1932 mL
5mM	0.4919 mL	2.4597 mL	4.9193 mL	9.8386 mL
10mM	0.2460 mL	1.2298 mL	2.4597 mL	4.9193 mL
20mM	0.1230 mL	0.6149 mL	1.2298 mL	2.4597 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.