25-Hydroxycholesterol
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $750 | Check With Us |
| 1g | $1250 | Check With Us |
| 5g | $3165 | Check With Us |
: Cat #: V31638 CAS #: 2140-46-7 Purity ≥ 99%
Description: 25-Hydroxycholesterol is a major metabolite of cholesterol produced by macrophages in response to the activation of Toll-like receptor (TLR).
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- Related Biological Data
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| Molecular Weight (MW) | 402.65 |
|---|---|
| Molecular Formula | C27H46O2 |
| CAS No. | 2140-46-7 |
| Synonyms | 25-Hydroxycholesterol |
| Protocol | In Vitro | The synthesis of 25-hydroxycholesterol is catalyzed by the enzyme cholesterol 25-hydroxylase, which uses cholesterol and molecular oxygen as substrates and NADPH as a cofactor. 25-hydroxycholesterol is a potent bioactive lipid in the innate and adaptive immune systems. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.4835 mL | 12.4177 mL | 24.8355 mL | 49.6709 mL |
| 5mM | 0.4967 mL | 2.4835 mL | 4.9671 mL | 9.9342 mL |
| 10mM | 0.2484 mL | 1.2418 mL | 2.4835 mL | 4.9671 mL |
| 20mM | 0.1242 mL | 0.6209 mL | 1.2418 mL | 2.4835 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.