Z-VAD-FMK
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $1450 | In Stock |
| 500mg | $2080 | In Stock |
| 1g | $3120 | In Stock |
: Cat #: V0028 CAS #: 187389-52-2 Purity ≥ 98%
Description: Z-VAD-FMK (Z-VAD(OMe)-FMK; Z-Val-Ala-Asp(OMe)-FMK), a peptide compound, is a novel, potent cell-permeable and irreversible (covalent) inhibitor of pan-caspase.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 467.49 |
|---|---|
| Molecular Formula | C22H30FN3O7 |
| CAS No. | 187389-52-2 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 93 mg/mL (198 mM)r |
| Water:<1 mg/mL (slightly soluble or insoluble)r | |
| Ethanol: 24 mg/mL warming (51 mM) | |
| SMILES Code | O=C(OC)C[C@H](NC([C@@H](NC([C@@H](NC(OCC1=CC=CC=C1)=O)C(C)C)=O)C)=O)C(CF)=O |
| Synonyms | Z-VAD(OMe)-FMK; Z-Val-Ala-Asp(OMe)-FMK; Z-VAD-FMK; Z VADFMK; ZVADFMK; |
| Protocol | In Vitro | Z-VAD(OMe)-FMK (Z-Val-Ala-Asp(OMe)-FMK) is a broad-spectrum caspase inhibitor, has been shown to inhibit the intracellular activation of caspase-like proteases. The injection of Z-VAD(OMe)-FMK suppresses the caspase-3 activity in lung tissues, and significantly decreases the number of terminal dUTP nick-end labeling-positive cells. |
|---|---|---|
| In Vivo | The survival rate of mice is prolonged significantly by the injection of Z-VAD(OMe)-FMK (Z-Val-Ala-Asp(OMe)-FMK). All mice succumbed to LPS within 30 hours. By contrast, the mice treated with Z-VAD(OMe)-FMK survive significantly longer and 27% of the mice survived more than 7 days. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.1391 mL | 10.6954 mL | 21.3908 mL | 42.7817 mL |
| 5mM | 0.4278 mL | 2.1391 mL | 4.2782 mL | 8.5563 mL |
| 10mM | 0.2139 mL | 1.0695 mL | 2.1391 mL | 4.2782 mL |
| 20mM | 0.1070 mL | 0.5348 mL | 1.0695 mL | 2.1391 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
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Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
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Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.