Z-IETD-FMK
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $2350 | Check With Us |
| 200mg | $3525 | Check With Us |
| 500mg | $5950 | Check With Us |
: Cat #: V0030 CAS #: 210344-98-2 Purity ≥ 98%
Description: Z-IETD-FMK (also known as Z-IE(OMe)TD(OMe)-FMK) is a novel, specific, selective, irreversible and cell permeable Caspase-8/9 inhibitor.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 654.68 |
|---|---|
| Molecular Formula | C30H43FN4O11 |
| CAS No. | 210344-98-2 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 91 mg/mL (138.99 mM)r |
| Water:<1 mg/mL (slightly soluble or insoluble)r | |
| Ethanol: <1 mg/mL | |
| SMILES Code | O=C(O)C[C@@H](C(CF)=O)NC([C@H]([C@H](O)C)NC([C@H](CCC(O)=O)NC([C@H]([C@@H](C)CC)NC(OCC1=CC=CC=C1)=O)=O)=O)=O |
| Synonyms | Z-IETD-FMK; MDK4982; MDK-4982; MDK 4982; Z-Ile-Glu(OMe)-Thr-Asp(OMe)-CH₂F; Z-IE(OMe)TD(OMe)-FMK; Granzyme B Inhibitor III; |
| Protocol | In Vitro | Z-IETD-FMK causes full inhibition only of the proapoptotic effect of TNFα with an IC50 of 0.46 μM. |
|---|---|---|
| In Vivo | Pharmacological inhibition of caspase-8 by z-IETD-FMK robustly reduces tumour outgrowth and this is closely associated with a reduction in the release of pro-inflammatory cytokines, IL-6, TNF-α, IL-18, IL-1α, IL-33, but not IL-1β. Furthermore, inhibition of caspase-8 reduces the recruitment of innate suppressive cells, such as myeloid-derived suppressor cells, but not of regulatory T cells to lungs of tumour-bearing mice. | |
| Animal model | T. cruzi-infected mice |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 1.5275 mL | 7.6373 mL | 15.2746 mL | 30.5493 mL |
| 5mM | 0.3055 mL | 1.5275 mL | 3.0549 mL | 6.1099 mL |
| 10mM | 0.1527 mL | 0.7637 mL | 1.5275 mL | 3.0549 mL |
| 20mM | 0.0764 mL | 0.3819 mL | 0.7637 mL | 1.5275 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.