Vitamin D2 (Ergocalciferol)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
25g	$500	To Be Confirmed
50g	$850	To Be Confirmed
100g	$1275	To Be Confirmed

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Cat #: V5286 CAS #: 50-14-6 Purity ≥ 98%

Description: Vitamin D2 (Ergocalciferol; Calciferol; Ercalciol) is one form of vitamin D with a double bond on the side chain, which differentiates it from Vitamin D3 (without a double bond on the side chain).

References: Sagar U Nigwekar, et al. Ergocalciferol and cholecalciferol in CKD. Am J Kidney Dis. 2012 Jul;60(1):139-56.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	396.65
Molecular Formula	C28H44O
CAS No.	50-14-6
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
SMILES Code	O[C@@H]1C/C(C(CC1)=C)=C/C=C2[C@]3([H])CC[C@H]([C@H](C)/C=C/[C@H](C)C(C)C)[C@@]3(C)CCC/2
Synonyms	Ergocalciferol; Viosterol; Vitamin D2; calciferol,

Protocol	In Vivo	At sacrifice, after 5 weeks of treatment, chickens receiving omeprazole alone or omeprazole in combination with either restricted food intake or Ergocalciferol weighed 15-25% less (P < 0.01) than chickens given vehicle or receiving reduced amount of food.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.5211 mL	12.6056 mL	25.2111 mL	50.4223 mL
5mM	0.5042 mL	2.5211 mL	5.0422 mL	10.0845 mL
10mM	0.2521 mL	1.2606 mL	2.5211 mL	5.0422 mL
20mM	0.1261 mL	0.6303 mL	1.2606 mL	2.5211 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.