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Tris(2,4-di-tert-butylphenyl)phosphate

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Tris(2,4-di-tert-butylphenyl)phosphate
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Cat #: V2316 CAS #: 95906-11-9 Purity ≥ 99%

Description: Tris(2,4-di-tert-butylphenyl)phosphate is a bioactive compound isolated from the leaves of Vitex negundo L with anti-inflammatory activity.

References: Vinuchakkaravarthy T, et al. Active compound from the leaves of Vitex negundo L. shows anti-inflammatory activity with evidence of inhibition for secretory Phospholipase A(2) through molecular docking. Bioinformation. 2011;7(4):199-206.

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Molecular Weight (MW)662.92
Molecular FormulaC42H63O4P
CAS No.95906-11-9
SynonymsTris(2,4-di-tert-butylphenyl)phosphate
ProtocolIn VitroTris(2,4-di-tert-butylphenyl)phosphate (50 mg/kg and 70 mg/kg) reduces the raw paw odema volume significantly. Tris(2,4-di-tert-butylphenyl)phosphate (TDTBPP; 50 mg/kg and 70 mg/kg) exhibits significant anti-inflammatory activity in carrageenan induced paw odema model.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM1.5085 mL7.5424 mL15.0848 mL30.1696 mL
5mM0.3017 mL1.5085 mL3.0170 mL6.0339 mL
10mM0.1508 mL0.7542 mL1.5085 mL3.0170 mL
20mM0.0754 mL0.3771 mL0.7542 mL1.5085 mL
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
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Concentration
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Volume
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Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.