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STO-609

This product is for research use only, not for human use. We do not sell to patients.

STO-609
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
250mg$1150In Stock
500mg$1750In Stock
1g$2625In Stock
email: [email protected]        [email protected]

Cat #: V2702 CAS #: 52029-86-4 Purity ≥ 98%

Description: STO-609 is a novel, potent, specific and cell-permeable inhibitor of the Ca2+/Calmodulin-dependent protein kinase kinase(CaM-KK) that inhibits the activities of recombinant CaM-KKα and CaM-KKβ isoforms with Ki values of 80 and 15 ng/ml, respectively, it also inhibits their autophosphorylation activities.

References: Tokumitsu H, et al. STO-609, a specific inhibitor of the Ca(2+)/calmodulin-dependent protein kinase kinase. J Biol Chem. 2002 May 3;277(18):15813-8.

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Molecular Weight (MW)314.30
Molecular FormulaC19H10N2O3
CAS No.52029-86-4
Storage-20℃ for 3 years in powder formrr
-80℃ for 2 years in solvent
Solubility In VitroDMSO: 5.6 mg/mLrr
Water: <1 mg/mLrr
Ethanol: <1 mg/mL
SMILES CodeO=C1C2=CC=CC3=C(C(O)=O)C=CC(C4=NC(C=CC=C5)=C5N41)=C32
SynonymsSTO-609; STO 609; STO609
ProtocolIn VitroSTO-609 inhibits the activities of recombinant CaM-KKα and CaM-KKβ isoforms, with Ki values of 80 and 15 ng/mL, respectively, and also inhibits their autophosphorylation activities. STO-609 is highly selective for CaM-KK without any significant effect on the downstream CaM kinases (CaM-KI and -IV), and the IC50 value of the compound against CaM-KII is 10 μg/mL. STO-609 inhibits constitutively active CaM-KKα as well as the wild-type enzyme. In transfected HeLa cells, STO-609 suppresses the Ca2+-induced activation of CaM-KIV in a dose-dependent manner. STO-609 significantly reduces the endogenous activity of CaM-KK in SH-SY5Y neuroblastoma cells at a concentration of 1μg/mL (80% inhibitory rate)
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM3.1817 mL15.9084 mL31.8167 mL63.6335 mL
5mM0.6363 mL3.1817 mL6.3633 mL12.7267 mL
10mM0.3182 mL1.5908 mL3.1817 mL6.3633 mL
20mM0.1591 mL0.7954 mL1.5908 mL3.1817 mL
Quality Control Documentation
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The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

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Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O
Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.