Smoothened Agonist (SAG)
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $1750 | Check With Us |
| 500mg | $2350 | Check With Us |
| 1g | $35250 | Check With Us |
: Cat #: V1338 CAS #: 912545-86-9 Purity ≥ 98%
Description: Smoothened Agonist (SAG), a benzothiophene analog, is a potent and cell-permeable Smoothened (Smo) agonist with EC50 of 3 nM in Shh-LIGHT2 cells.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 490.06 |
|---|---|
| Molecular Formula | C28H28ClN3OS |
| CAS No. | 912545-86-9 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 71 mg/mL (134.8 mM)r |
| Water: N/Ar | |
| Ethanol: 40 mg/mL (75.9 mM) | |
| Synonyms | SAG free base; SAG; SAG (cyclopamine antagonist);SAG (Smo agonist) |
| Protocol | In Vivo | SAG (15-20 mg/kg; i.p.) induces pre-axial polydactyly prevalently in a dose-dependent manner in mice |
|---|---|---|
| Animal model | Pregnant C57BL/6J mice | |
| Dosages | 15, 17, 20 mg/kg | |
| Administration | A single i.p. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.0406 mL | 10.2028 mL | 20.4057 mL | 40.8113 mL |
| 5mM | 0.4081 mL | 2.0406 mL | 4.0811 mL | 8.1623 mL |
| 10mM | 0.2041 mL | 1.0203 mL | 2.0406 mL | 4.0811 mL |
| 20mM | 0.1020 mL | 0.5101 mL | 1.0203 mL | 2.0406 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.