SB225002
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $595 | In Stock |
| 500mg | $1050 | In Stock |
| 1g | $1575 | In Stock |
: Cat #: V1490 CAS #: 182498-32-4 Purity ≥ 98%
Description: SB225002 (SB 225002; SB-225002) is a novel, potent, and selective non-peptide antagonist of chemokine receptor CXCR2 with potential anti-inflammatory activity.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 352.14 |
|---|---|
| Molecular Formula | C13H10BrN3O4 |
| CAS No. | 182498-32-4 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 70 mg/mL (198.7 mM)r |
| Water:<1 mg/mLr | |
| Ethanol: 3 mg/mL warmed (8.5 mM) | |
| Solubility In Vivo | 2% DMSO+Castor oil: 10mg/mL |
| Synonyms | SB225002; SB 225002; SB225002; Chemical Name: 1-(2-bromophenyl)-3-(2-hydroxy-4-nitrophenyl)urea SMILES Code: O=C(NC1=CC=C([N+]([O-])=O)C=C1O)NC2=CC=CC=C2Br Exact Mass: 350.98547 |
| Protocol | In Vitro | SB225002 (SB 225002) is an antagonist of 125I-IL-8 binding to CXCR2 with an IC50=22 nM. SB225002 shows >150-fold selectivity over CXCR1 and four other 7-TMRs tested. SB225002 is a potent antagonist of rabbit CXCR2, inhibiting rabbit PMN chemotaxis in response to optimal concentrations of human IL-8 or GROα (IC50 values of 30 and 70 nM, respectively. In these cells (PMN, HL60, CXCR1-RBL-2H3), SB225002 produces a concentration-dependent inhibition of both IL-8- and GROα-mediated calcium mobilization with IC50 values of 8 and 10 nM, respectively. In 3ASubE cells stably transfected with CXCR2, SB 225002 dose-dependently inhibits calcium mobilization induced by both GROα and IL-8, with IC50 values of 20 and 40 nM, respectively |
|---|---|---|
| In Vivo | SB225002 (SB 225002) selectively blocks IL-8-induced neutrophil margination in rabbits |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.8398 mL | 14.1989 mL | 28.3978 mL | 56.7956 mL |
| 5mM | 0.5680 mL | 2.8398 mL | 5.6796 mL | 11.3591 mL |
| 10mM | 0.2840 mL | 1.4199 mL | 2.8398 mL | 5.6796 mL |
| 20mM | 0.1420 mL | 0.7099 mL | 1.4199 mL | 2.8398 mL |
The molarity calculator equation
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
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Average weight of animals g
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Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.