SAG hydrochloride
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $1500 | To Be Confirmed |
| 500mg | $2150 | To Be Confirmed |
| 1g | $3225 | To Be Confirmed |
: Cat #: V7879 CAS #: 2095432-58-7 Purity ≥ 98%
Description: Smoothened Agonist (SAG) HCl is a potent and cell-permeable Smoothened (Smo) agonist with EC50 of 3 nM in Shh-LIGHT2 cells.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 526.52 |
|---|---|
| Molecular Formula | C28H29Cl2N3OS |
| CAS No. | 2095432-58-7 |
| Protocol | In Vitro | SAG (0.1 nM-100 μM; 30 h) induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM and then inhibits expression at higher concentrations |
|---|---|---|
| In Vivo | SAG (1.0 mM) induces more osteogenesis mainly at the defect borders and a significant increase in BV/TV at the eight week timepoint in CD-1 mice | |
| Animal model | Pregnant C57BL/6J mice | |
| Dosages | 15, 17, 20 mg/kg | |
| Administration | A single i.p. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 1.8993 mL | 9.4963 mL | 18.9926 mL | 37.9853 mL |
| 5mM | 0.3799 mL | 1.8993 mL | 3.7985 mL | 7.5971 mL |
| 10mM | 0.1899 mL | 0.9496 mL | 1.8993 mL | 3.7985 mL |
| 20mM | 0.0950 mL | 0.4748 mL | 0.9496 mL | 1.8993 mL |
The molarity calculator equation
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.