Polymyxin B sulphate

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
5g	$180	Check With Us
10g	$300	Check With Us
20g	$450	Check With Us

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Cat #: V1863 CAS #: 1405-20-5 Purity ≥ 98%

Description: Polymyxin B (also known as Aerosporin, PMB, Poly-RX) is an antibiotic and a cationic surfactant primarily used for resistant gram-negative infections. Polymyxin B sulfate is a mixture of polymyxins B1 and B2, obtained from Bacillus polymyxa strains. They are basic polypeptides of about eight amino acids and have cationic detergent action on cell membranes. Polymyxin B is used for infections with gram-negative organisms, but may be neurotoxic and nephrotoxic.

References: [1]. Danner RL, et al. Purification, toxicity, and antiendotoxin activity of polymyxin B nonapeptide. Antimicrob Agents Chemother. 1989 Sep;33(9):1428-34.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	1301.56
Molecular Formula	C56H98N16O13.H2SO4
CAS No.	1405-20-5
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: <1 mg/mLr
	Water: 100 mg/mL (76.83 mM)r
	Ethanol: <1 mg/mL
SMILES Code	CCC(C)CCCCC(=O)NC(CCN)C(=O)NC(C(C)O)C(=O)NC(CCN)C(=O)NC1CCNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC1=O)CCN)CC2=CC=CC=C2)CC(C)C)CCN)CCN)C(C)O.OS(=O)(=O)O
Synonyms	Aerosporin, PMB, Poly-RX; KS-1428; KS 1428; KS1428; Polymyxin B Sulfate; Polymyxin B;

Protocol	In Vitro	Polymyxin B Sulfate shows antibacterial activities with MICs of 0.5 mg/l for E. coli strain IH3080.
	In Vivo	Polymyxin B Sulfate (0.5-120 mg/kg; s.c.) shows antibacterial activities in thigh or lung infection mouse model. Polymyxin B Sulfate (2 mg/kg, s.c.) shows potent in mouse bactericidal effect against E. coli strain IH3080.
	Animal model	Eight-week-old, 24-30 g, female Swiss mice
	Dosages	0.5-120 mg/kg for thigh infection model; 5-120 mg/kg for lung infection mode
	Administration	S.c.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	0.7683 mL	3.8415 mL	7.6831 mL	15.3662 mL
5mM	0.1537 mL	0.7683 mL	1.5366 mL	3.0732 mL
10mM	0.0768 mL	0.3842 mL	0.7683 mL	1.5366 mL
20mM	0.0384 mL	0.1921 mL	0.3842 mL	0.7683 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.