PMSF

This product is for research use only, not for human use. We do not sell to patients.

PMSF
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
25g$250Check With Us
50g$450Check With Us
100mg$675Check With Us

Cat #: V0701 CAS #: 329-98-6 Purity ≥ 98%

Description: PMSF (full name: Phenylmethanesulfonyl fluoride) is a potent and irreversible inhibitor of serine/cysteine protease (e.g. thrombin, trypsin and chymotrypsin) with potential analgesic effects.

References: Sekar, M.C. and B.D. Roufogalis, Differential effects of phenylmethanesulfonyl fluoride (PMSF) on carbachol and potassium stimulated phosphoinositide turnover and contraction in longitudinal smooth muscle of guinea pig ileum. Cell Calcium, 1984. 5(3): p.

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Molecular Weight (MW)174.19
Molecular FormulaC7H7FO2S
CAS No.329-98-6
Storage-20℃ for 3 years in powder formr
-80℃ for 2 years in solvent
Solubility In VitroDMSO: 35 mg/mL (200.9 mM)r
Water:<1 mg/mLr
Ethanol: 15 mg/mL (86.1 mM)
SMILES CodeO=S(CC1=CC=CC=C1)(F)=O
SynonymsPhenylmethylsulfonyl Fluoride; PMSF; Phenylmethylsulfonyl fluoride; Benzylsulfonyl fluorid;
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM5.7409 mL28.7043 mL57.4086 mL114.8172 mL
5mM1.1482 mL5.7409 mL11.4817 mL22.9634 mL
10mM0.5741 mL2.8704 mL5.7409 mL11.4817 mL
20mM0.2870 mL1.4352 mL2.8704 mL5.7409 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.