Nutlin-3a
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $1550 | Check With Us |
| 1g | $2550 | Check With Us |
| 5g | $6450 | Check With Us |
: Cat #: V0042 CAS #: 675576-98-4 Purity ≥ 98%
Description: Nutlin-3a, the active enantiomer of Nutlin-3, is a novel and potent p53/MDM2 (murine double minute) protein protein interaction inhibitor with an IC50 of 90 nM in cell-free assays.
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| Molecular Weight (MW) | 581.49 |
|---|---|
| Molecular Formula | C30H30Cl2N4O4 |
| CAS No. | 675576-98-4 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 100 mg/mL (171.97 mM)r |
| Water:<1 mg/mL (slightly soluble or insoluble)r | |
| Ethanol: N/A | |
| Solubility In Vivo | 5% DMSO+55% PEG 300+ddH2O: 8 mg/mL |
| SMILES Code | O=C1NCCN(C(N2[C@H](C3=CC=C(Cl)C=C3)[C@H](C4=CC=C(Cl)C=C4)N=C2C5=CC=C(OC)C=C5OC(C)C)=O)C1 |
| Synonyms | (-)-Nutlin 3, (-)-Nutlin-3; SML 0580; SML0580; SML-0580; Nutlin3a; Nutlin 3a; Nutlin-3a; |
| Protocol | In Vitro | Nutlin-3a is a therapeutic which inhibits MDM2, activates wild-type p53, and induces apoptosis-as a therapeutic compound for TP53 wild-type ovarian carcinomas. Three cell lines (HOC-7, OVCA429 and A2780) with wild-type TP53 are highly sensitive to Nutlin-3a (IC50=4 to 6 μM). SKOV3 cells have an IC50 of 38 μM to Nutlin-3a. The two remaining ovarian clear cell lines (TOV21G and OVAS), both with TP53 wild-type, are relatively more sensitive to growth inhibition with Nutlin-3a (IC50=14 and 25 μm respectively) than the TP53 mutant cell lines. |
|---|---|---|
| In Vivo | Nutlin-3a is efficacious in all models with average tumor growth inhibition ≥98%. Nutlin-3a suppresses xenograft growth in a dose-dependent fashion with the highest dose (200 mg/kg) showing a substantial tumor shrinkage (eight partial and one full regressions). The established SJSA-1 and MHM osteosarcoma xenografts with Nutlin-3a causes extensive tumor regression. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 1.7197 mL | 8.5986 mL | 17.1972 mL | 34.3944 mL |
| 5mM | 0.3439 mL | 1.7197 mL | 3.4394 mL | 6.8789 mL |
| 10mM | 0.1720 mL | 0.8599 mL | 1.7197 mL | 3.4394 mL |
| 20mM | 0.0860 mL | 0.4299 mL | 0.8599 mL | 1.7197 mL |
The molarity calculator equation
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
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Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.