ML-7 | CAS 109376-83-2

ML-7

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
25mg	$150	To Be Confirmed
50mg	$250	To Be Confirmed
100mg	$450	To Be Confirmed
250mg	$800	To Be Confirmed
500mg	$1250	To Be Confirmed

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Cat #: V4010 CAS #: 109376-83-2 Purity ≥ 98%

Description: ML-7 (ML7) is a potent Myosin light chain kinase (MLCK) inhibitor with IC50 of 300 nM and IC50>10 μM for MLCK and TRPC6 channel respectively. Myosin light chain kinase (MLCK) plays a pivotal role in regulation of cellular functions, the evidence often relying on the effects of extracelluarly administered drugs such as ML-9. Here we report that this compound exerts non-specific inhibitory actions on the TRPC6 channel, a transient receptor potential (TRP) protein.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	416.28
Molecular Formula	C15H17IN2O2S
CAS No.	109376-83-2
Storage	-20℃ for 3 years in powder form
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: ≥ 30mg/mL
	Water: N/A
	Ethanol: N/A
SMILES Code	O=S(N1CCNCCC1)(C2=C3C=CC=C(I)C3=CC=C2)=O
Synonyms	ML-7; ML-7 ML7.

Protocol	In Vitro	ML-7 hydrochloride inhibits rabbit portal vein α1-adrenoceptor NSCC with IC50 of 0.8 μM[1]. The myosin light chain kinase (MLCK) inhibitor ML-7 hydrochloride (3 μ M and 10 μM) also attenuates the Dexmedetomidine (DMT)-induced contraction (p<0.05 versus control)[2].
Protocol	In Vivo	In sham operated animals Evans Blue extravasation is not different between ML-7 hydrochloride and vehicle group (sham+vehicle: 0.26±0.02 OD/g; sham+ML-7: 0.26±0.02 OD/g). After CCI inhibition of MLCK with ML-7 results in a significant lower amount of intracerebral Evans Blue compared to vehicle treated animals (CCI+vehicle: 0.42±0.04 OD/g; CCI+ML-7: 0.35±0.05 OD/g, p=0.048)[3].

These protocols are for reference only. InvivoChem does not independently validate these methods.

Related Biological Data

Effects of ML-9 and ML-7 on single TRPC6 channels. Br J Pharmacol. 2007 Sep;152(1):122-31.

Activation mode of TRPC6 did not affect the efficacy of ML-9 and ML-7.

Enhancing actions of ML-compounds on macroscopic TRPC7 current (ITRPC7). Conventional whole-cell recording at −60 mV with Ca2+-free external solution. Br J Pharmacol. 2007 Sep;152(1):122-31.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.4022 mL	12.0111 mL	24.0223 mL	48.0446 mL
5mM	0.4804 mL	2.4022 mL	4.8045 mL	9.6089 mL
10mM	0.2402 mL	1.2011 mL	2.4022 mL	4.8045 mL
20mM	0.1201 mL	0.6006 mL	1.2011 mL	2.4022 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.