Methoxy-PMS

This product is for research use only, not for human use. We do not sell to patients.

Methoxy-PMS
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Size Price Stock
500mg$300Check With Us
1g$500Check With Us
5g$1265Check With Us

Cat #: V29766 CAS #: 65162-13-2 Purity ≥ 99%

Description: Methoxy-PMS (1-Methoxy PMS; 1-Methoxyphenazine methosulfate) is stable electron-transport mediator between NAD(P)H and tetrazolium dyes.

References: De Niz M et al. Tools for mass screening of G6PD deficiency: validation of the WST8/1-methoxy-PMS enzymatic assay in Uganda. Malar J. 2013 Jun 19;12:210.

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Molecular Weight (MW)336.36
Molecular FormulaC15H16N2O5S
CAS No.65162-13-2
SynonymsMethoxy-PMS; 1-Methoxy PMS; 1-Methoxyphenazine methosulfate
ProtocolIn VitroMethoxy-PMS has no cytotoxicity in the cell culture media. Methoxy-PMS receives an electron from NADH or NADPH at the membrane or inside of the cell and passes the electron to the WST-8 that is around the outer cell membrane.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM2.9730 mL14.8650 mL29.7301 mL59.4601 mL
5mM0.5946 mL2.9730 mL5.9460 mL11.8920 mL
10mM0.2973 mL1.4865 mL2.9730 mL5.9460 mL
20mM0.1487 mL0.7433 mL1.4865 mL2.9730 mL
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
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Concentration
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Volume
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Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
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Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.