Lysophosphatidic acid

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Lysophosphatidic acid
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Size Price Stock
100mg$750Check With Us
250mg$1450Check With Us
500mg$2175Check With Us

Cat #: V40955 CAS #: 325465-93-8 Purity ≥ 99%

Description: Lysophosphatidic acid is an endogenous agonist of the lysophospholipid receptors LPA1 and LPA2, a glycerophospholipid signaling ligand molecule and ligand activator for EDG-2, EDG-4, and EDG-7.

References: Lapierre DM, et, al. Lysophosphatidic acid signals through multiple receptors in osteoclasts to elevate cytosolic calcium concentration, evoke retraction, and promote cell survival. J Biol Chem. 2010 Aug 13;285(33):25792-801.

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Molecular Weight (MW)458.50
Molecular FormulaC21H40NaO7P
CAS No.325465-93-8
ProtocolIn Vitro1-Oleoyl lysophosphatidic acid (0.1-10 μM) sodium elicits an acute rise of [Ca2+]i in rat and rabbit osteoclasts. 1-Oleoyl lysophosphatidic acid (5 μM) sodium induces retraction of osteoclast lamellipodia
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM2.1810 mL10.9051 mL21.8103 mL43.6205 mL
5mM0.4362 mL2.1810 mL4.3621 mL8.7241 mL
10mM0.2181 mL1.0905 mL2.1810 mL4.3621 mL
20mM0.1091 mL0.5453 mL1.0905 mL2.1810 mL
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
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Volume
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Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.