Linoleylethanolamide

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
500mg	$725	Check With Us
1g	$1125	Check With Us
5g	$2925	Check With Us

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Cat #: V5025 CAS #: 68171-52-8 Purity ≥ 98%

Description: Linoleylethanolamide is a novel and potent endocannabinoid agent that acts by binding to TRPV1 (Ki = 5.60 uM), increasing ERK phosphorylation and AP-1 dependent transcription in CB-receptor in an independent manner.

References: Wang X, et al. X. Synthesis of linoleoyl ethanolamide. J Oleo Sci. 2013;62(6):427-433.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	323.51
Molecular Formula	C20H37NO2
CAS No.	68171-52-8
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent

Protocol	In Vitro	Linoleoyl ethanolamide competitively inhibits the hydrolysis of anandamide and may be involved in the regulation of food intake by selective prolongation of feeding latency and post-meal interval
Protocol	In Vivo	Linoleoyl ethanolamide (0.1 mg-0.2 mg; challenged by painting the inner and outer surfaces of both sides of their ears) suppresses DNFB-induced ear swelling in BALB/c mice

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.0911 mL	15.4555 mL	30.9109 mL	61.8219 mL
5mM	0.6182 mL	3.0911 mL	6.1822 mL	12.3644 mL
10mM	0.3091 mL	1.5455 mL	3.0911 mL	6.1822 mL
20mM	0.1546 mL	0.7728 mL	1.5455 mL	3.0911 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.