K02288 | CAS 1431985-92-0

K02288

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$650	In Stock
500mg	$975	In Stock
1g	$1460	In Stock

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Cat #: V1374 CAS #: 1431985-92-0 Purity ≥ 98%

Description: K02288 (K-02288; K 02288) is a potent, 2-aminopyridine-based and selective small molecule inhibitor of the BMP (bone morphogenetic protein) signaling pathway with the potential to treat the progressively debilitating musculoskeletal disease fibrodysplasia ossificans progressiva (FOP).

References: Kerr G, et al. A small molecule targeting ALK1 prevents Notch cooperativity and inhibits functional angiogenesis. Angiogenesis. 2015 Apr;18(2):209-17.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	352.38
Molecular Formula	C20H20N2O4
CAS No.	1431985-92-0
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 70 mg/mL (198.6 mM)r
	Water: <1 mg/mLr
	Ethanol: <1 mg/mL
SMILES Code	OC1=CC=CC(C2=CC(C3=CC(OC)=C(OC)C(OC)=C3)=C(N)N=C2)=C1
Synonyms	K02288; K 02288; K-02288 Chemical Name: 3-(6-amino-5-(3,4,5-trimethoxyphenyl)pyridin-3-yl)phenol

Protocol	In Vitro	K02288 reduces a robust phosphorylation of Smad1/5/8 induced by BMP4 stimulation, with an apparent IC50 of 100 nM. K02288 causes near complete inhibition of Smad2 phosphorylation at 0.5 µM[1]. K02288 binds to ALK1 in an ATP-mimetic fashion with two hydrogen bonds to the kinase hinge. K02288 also inhibits BMP9-ALK1 signalling, and induces a hypersprouting phenotype in HUVECs
Protocol	In Vivo	K02288 (1 µM) induces dysfunctional angiogenesis in a chick embryo CAM model

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.8378 mL	14.1892 mL	28.3785 mL	56.7569 mL
5mM	0.5676 mL	2.8378 mL	5.6757 mL	11.3514 mL
10mM	0.2838 mL	1.4189 mL	2.8378 mL	5.6757 mL
20mM	0.1419 mL	0.7095 mL	1.4189 mL	2.8378 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.