josamycin | CAS 16846-24-5 (Josamycin)

josamycin

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1g	$550	Check With Us
5g	$1390	Check With Us

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Cat #: V3558 CAS #: 16846-24-5 (Josamycin) Purity ≥ 98%

Description: Josamycin (BRN-1677122; CCRIS 8511; EN-141) is a macrolide antibiotic that exhibits antimicrobial activity against a wide spectrum of pathogens, such as bacteria. The dissociation constant Kd from ribosome for Josamycin is 5.5 nM. It is synthesized from strains of Streptomyces narbonensis var.

References: Lovmar M, et al. Kinetics of macrolide action: the Josamycin and erythromycin cases. J Biol Chem. 2004 Dec 17;279(51):53506-15.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	828.006
Molecular Formula	C42H69NO15
CAS No.	16846-24-5 (Josamycin)
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
SMILES Code	O([C@@H]1[C@H]([C@@H](CC(O[C@@H](CC=CC=C[C@@H]([C@@H](C[C@@H]1CC=O)C)O)C)=O)OC(C)=O)OC)[C@H]1[C@@H]([C@H]([C@H](O[C@H]2C[C@@]([C@@H](OC(CC(C)C)=O)[C@@H](O2)C)(C)O)[C@H](O1)C)N(C)C)
Synonyms	Josamycin; BRN-1677122; CCRIS 8511; BRN 1677122; BRN1677122; Josamycin hydrochloride; Leucomycin A3 hydrochloride;

Protocol	In Vitro	Studies show that the average lifetime on the ribosome is 3 h for Josamycin and that the dissociation constants for Josamycin binding to the ribosome is 5.5 nM. Josamycin slows down formation of the first peptide bond of a nascent peptide in an amino acid-dependent way and completely inhibits formation of the second or third peptide bond, depending on peptide sequence at a saturating drug concentration, synthesis of fulllength proteins is completely shut down by Josamycin. At a saturating drug concentration, synthesis of fulllength proteins is completely shut down by Josamycin.
Protocol	In Vivo	Blood and tissue levels of Josamycin after oral administration are 200 mg/kg to rabbits. Tissue levels are generally much higher than the blood levels, and 3 h after the administration, when the blood level is very low, the tissue levels are rather higher than those 1 h after the dose. One hour after the medication, the level in the lungs is the highest of all the tissue levels.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.2077 mL	6.0386 mL	12.0772 mL	24.1544 mL
5mM	0.2415 mL	1.2077 mL	2.4154 mL	4.8309 mL
10mM	0.1208 mL	0.6039 mL	1.2077 mL	2.4154 mL
20mM	0.0604 mL	0.3019 mL	0.6039 mL	1.2077 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.