JIB-04 Z-isomer

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
250mg	$1250	Check With Us
500mg	$1950	Check With Us
1g	$2925	Check With Us

: [email protected] [email protected]

Cat #: V22899 CAS #: 199596-24-2 Purity ≥ 99%

Description: JIB-04 Z-isomer, formerly known as NSC 693627, is the Z-configurational isomer of JIB-04 which is a potent and pan-selective Jumonji histone demethylase inhibitor with IC50s of 230, 340, 855, 445, 435, 1100, and 290 nM for JARID1A, JMJD2E, JMJD3, JMJD2A, JMJD2B, JMJD2C, and JMJD2D, respectively..

References: Wang L, et al. A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth. Nat Commun. 2013;4:2035.

Top Publications Citing Invivochem Products

Product Catalog 2022

Guide to Product Handling

Publications Citing InvivoChem Products

Product Promise

Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	308.76
Molecular Formula	C17H13ClN4
CAS No.	199596-24-2
SMILES Code	ClC1=CN=C(N/N=C(C2=CC=CC=C2)\C3=NC=CC=C3)C=C1
Synonyms	JIB-04 Z-isomer, NSC-693627; NSC 693627; NSC693627

Protocol	In Vitro	JIB-04 inhibits the demethylase activity of Jumonji enzymes in vitro and in cells, without affecting other α-ketoglutarate dependent hydroxylases or histone modifying enzymes .

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.2388 mL	16.1938 mL	32.3876 mL	64.7752 mL
5mM	0.6478 mL	3.2388 mL	6.4775 mL	12.9550 mL
10mM	0.3239 mL	1.6194 mL	3.2388 mL	6.4775 mL
20mM	0.1619 mL	0.8097 mL	1.6194 mL	3.2388 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.