Incyclinide | CAS 15866-90-7

Incyclinide

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
100mg	$1250	Check With Us
250mg	$1950	Check With Us
500mg	$2925	Check With Us

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Cat #: V4106 CAS #: 15866-90-7 Purity ≥ 98%

Description: Incyclinide (formerly known as CMT-3 and COL-3; 6-demethyl, 6-deoxy, 4-dedimethylamino TC; a chemically modified tetracycline) is a matrix metalloproteinase (MMP) inhibitor, it acts by inducing extracellular matrix degradation, and inhibiting angiogenesis, tumor growth and invasion, and metastasis.

References: Lee HM, et al. CMT-3, a non-antimicrobial tetracycline (TC), inhibits MT1-MMPactivity: relevance to cancer. Curr Med Chem. 2001 Feb;8(3):257-60.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	371.34
Molecular Formula	C19H17NO7
CAS No.	15866-90-7
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: ≥ 100 mg/mLr
	Water: N/Ar
	Ethanol: N/A
SMILES Code	O=C(C1=C(O)C[C@@](C[C@@]2([H])C(C(C3=C(O)C=CC=C3C2)=O)=C4O)([H])[C@@]4(O)C1=O)N
Synonyms	COL3; COL-3; COL 3; CMT-3; CMT 3; CMT3; Incyclinide; 4-dedimethylamino sancycline; Chemically modified tetracycline-3. Trade name: Metastat.

Protocol	In Vitro	Incyclinide has been shown to experimentally suppress prostate cancer, colon adenocarcinoma and melanoma invasiveness in cell culture. Adding incyclinide at final concentrations of 5 to 20 μM inhibits MT1-MMP gelatinolytic and caseinolytic activity, blocks MT1-MMPactivation of pro-MMP-2, and decreases invasiveness of HT-1080 fibrosarcoma cells.
Protocol	In Vivo	Incyclinide inhibits tooth movement in the rat, probably by reducing the number of osteoclasts at the compression side. This might be due to induction of apoptosis in activated osteoclasts or reduced osteoclast migration. Reduced MMP activity by incyclinide might also directly inhibit degradation of the organic bone matrix.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.6929 mL	13.4647 mL	26.9295 mL	53.8590 mL
5mM	0.5386 mL	2.6929 mL	5.3859 mL	10.7718 mL
10mM	0.2693 mL	1.3465 mL	2.6929 mL	5.3859 mL
20mM	0.1346 mL	0.6732 mL	1.3465 mL	2.6929 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.