GSTO1-IN-1
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $739 | Check With Us |
| 1g | $1150 | Check With Us |
| 5g | $2910 | Check With Us |
: Cat #: V4571 CAS #: 568544-03-6 Purity ≥ 98%
Description: GSTO1-IN-1, a chloroacetamide-containing compound, is a novel and potent glutathione S-transferase omega 1 (GSTO1) inhibitor with an IC50 of 31 nM.
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- Related Biological Data
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| Molecular Weight (MW) | 311.18 |
|---|---|
| Molecular Formula | C₁₀H₁₂Cl₂N₂O₃S |
| CAS No. | 568544-03-6 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| SMILES Code | O=C(NC1=CC=C(Cl)C(S(=O)(N(C)C)=O)=C1)CCl |
| Synonyms | GSTO1-1 inhibitor C1-27 |
| Protocol | In Vitro | GSTO1-IN-1 (C1-27) potently inhibits GSTO1 enzyme activity with an IC50 value of 31 nM. GSTO1-IN-1 also potently competes with 5-chloromethylfluorescein diacetate (CMFDA) for binding to recombinant protein, as well as endogenous GSTO1 in the milieu of a soluble proteome. HCT116 cells treated with GSTO1-IN-1 also show a decrease in cell viability in a dose-dependent manner. GSTO1-IN-1 inhibits the clonogenic survival of HCT116 cells at sub-micromolar concentrations. |
|---|---|---|
| In Vivo | To test whether GSTO1-IN-1 had in vivo efficacy, its effects are evaluated in a human colon cancer cell line xenograft model. GSTO1-IN-1 (20-45 mg/kg) is administered as a single agent to nude mice bearing HCT116 xenografts. After 5 weeks of treatment, tumor growth is significantly inhibited in GSTO1-IN-1-treated mice compared with the vehicle-treated group (P<0.05). GSTO1-IN-1 treatment is generally well tolerated by mice up to 45 mg/kg, with no overt signs of toxicity and no significant variations in average body weights throughout the duration of the study. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.2136 mL | 16.0679 mL | 32.1357 mL | 64.2715 mL |
| 5mM | 0.6427 mL | 3.2136 mL | 6.4271 mL | 12.8543 mL |
| 10mM | 0.3214 mL | 1.6068 mL | 3.2136 mL | 6.4271 mL |
| 20mM | 0.1607 mL | 0.8034 mL | 1.6068 mL | 3.2136 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
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Volume
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Molecular Weight*
The dilution calculator equation
Concentration(start)
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Volume(start)
=
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Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
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Volume(start)
V1
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C2
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Volume(final)
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.