GSK2256294A | CAS 1142090-23-0

GSK2256294A

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
250mg	$1850	To Be Confirmed
500mg	$2650	To Be Confirmed
1g	$3975	To Be Confirmed

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Cat #: V2720 CAS #: 1142090-23-0 Purity ≥ 98%

Description: GSK2256294A (also known as GSK-2256294) is a novel, potent, selective inhibitor of recombinant human, rat and mouse sEH (Soluble epoxide hydrolase) (IC50 = 27 pM) with the potential to be used for endothelial dysfunction or abnormal tissue repair, such as diabetes, wound healing or COPD.

References: Podolin PL, et al. In vitro and in vivo characterization of a novel soluble epoxide hydrolase inhibitor. Prostaglandins Other Lipid Mediat. 2013 Jul-Aug;104-105:25-31.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	447.47
Molecular Formula	C21H24F3N7O
CAS No.	1142090-23-0
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: ≥ 50 mg/mLr
	Water: N/Ar
	Ethanol: N/A
SMILES Code	O=C([C@H]1C[C@@H](NC2=NC(C)=NC(NC)=N2)CCC1)NCC3=CC=C(C#N)C=C3C(F)(F)F
Synonyms	GSK2256294A; GSK2256294; GSK-2256294A; GSK-2256294; GSK 2256294A; GSK 2256294

Protocol	In Vitro	GSK2256294A (300 pM-10 μM; 2 h) inhibits the conversion of 14,15-EET-d11 to 14,15-DHET-d11 in human, rat and mouse whole blood.
Protocol	In Vivo	GSK2256294A (30 mg/kg; p.o. twice daily for 8 days) decreases pulmonary inflammation in cigarette smoke-exposed mice. GSK2256294A (5-30 mg/kg; p.o. twice daily 5 days/week for two weeks) inhibits the generation and maintenance of pulmonary inflammation in cigarette smoke-exposed mice.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.2348 mL	11.1739 mL	22.3479 mL	44.6957 mL
5mM	0.4470 mL	2.2348 mL	4.4696 mL	8.9391 mL
10mM	0.2235 mL	1.1174 mL	2.2348 mL	4.4696 mL
20mM	0.1117 mL	0.5587 mL	1.1174 mL	2.2348 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.