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Givinostat HCl monohydrate (ITF-2357; Gavinostat))

This product is for research use only, not for human use. We do not sell to patients.

Givinostat HCl monohydrate (ITF-2357; Gavinostat))
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
100mg$580In Stock
250mg$1180In Stock
500mg$1770In Stock
email: [email protected]        [email protected]

Cat #: V0289 CAS #: 732302-99-7 Purity ≥ 98%

Description: Givinostat (formerly ITF2357; Gavinostat) is a potent and orally bioavailable inhibitor of both class I and class II histone deacetylase (HDAC) with potential anticancer activity.

References: Li S, et al. Specific inhibition of histone deacetylase 8 reduces gene expression and production of proinflammatory cytokines in vitro and in vivo. J Biol Chem. 2015 Jan 23;290(4):2368-78.

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Molecular Weight (MW)475.97
Molecular FormulaC24H27N3O4.HCl.H2O
CAS No.732302-99-7
Storage-20℃ for 3 years in powder formr
-80℃ for 2 years in solvent
Solubility In VitroDMSO: 95 mg/mL (199.6 mM)r
Water: <1 mg/mLr
Ethanol: 3 mg/mL (6.3 mM)
Solubility In Vivo30% propylene glycol, 5% Tween 80, 65% D5W: 30 mg/mL
SynonymsITF2357; ITF 2357; ITF-2357; Givinostat ; gavinostat; ITF2357 HCl; ITF2357 hydrochloride; Givinostat HCl Chemical Name: (6-((diethylamino)methyl)naphthalen-2-yl)methyl (4-(hydroxycarbamoyl)phenyl)carbamate hydrochloride hydrate InChi Key: FKGKZBBDJSKCIS-UHFFFAOYSA-N InChi Code: InChI=1S/C24H27N3O4.ClH.H2O/c1-3-27(4-2)15-17-5-7-21-14-18(6-8-20(21)13-17)16-31-24(29)25-22-11-9-19(10-12-22)23(28)26-30;;/h5-14,30H,3-4,15-16H2,1-2H3,(H,25,29)(H,26,28);1H;1H2 SMILES Code: ONC(C1=CC=C(C=C1)NC(OCC2=CC3=C(C=C2)C=C(CN(CC)CC)C=C3)=O)=O.[H]Cl.[H]O[H]
ProtocolIn VitroGivinostat (ITF2357) suppresses total LPS-induced IL-1β production robustly compared with the reduction by ITF3056. At 25, 50, and 100 nM, Givinostat reduced IL-1β secretion more than 70%. Givinostat (ITF2357) suppresses the production of IL-6 in PBMCs stimulated with TLR agonists as well as the combination of IL-12 plus IL-18. IL-6 secretion decreases to 50% at 50 nM Givinostat (ITF2357), but at 100 and 200 nM, there is no reduction
In VivoGivinostat (ITF2357) at 10 mg/kg is used as a positive control and, as expected, reduced serum TNFα by 60%. Strikingly, pretreatment of ITF3056 starting at 0.1 mg/kg significantly reduces the circulating TNFα by nearly 90%. To achieve a significant increase in serum IL-1β production, a higher dose of LPS is injected (10 mg/kg), and blood is collected after 4 h. Similarly, when pretreated with lower doses of Givinostat (ITF2357) (1 or 5 mg/kg), there is a 22% reduction for 1 mg/kg and 40% for 5 mg/kg
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM2.1010 mL10.5049 mL21.0097 mL42.0195 mL
5mM0.4202 mL2.1010 mL4.2019 mL8.4039 mL
10mM0.2101 mL1.0505 mL2.1010 mL4.2019 mL
20mM0.1050 mL0.5252 mL1.0505 mL2.1010 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

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Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O
Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.