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freselestat

This product is for research use only, not for human use. We do not sell to patients.

freselestat
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
100mg$2650To Be Confirmed
200mg$3975To Be Confirmed
500mg$6705To Be Confirmed
email: [email protected]        [email protected]

Cat #: V4184 CAS #: 208848-19-5 Purity ≥ 98%

Description: Freselestat (also known as ONO-6818) is a novel, potent and selective inhibitor of Human Neutrophil Elastase (HNE).

References: Am J Respir Crit Care Med. 2002 Aug 15;166(4):496-500.

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Molecular Weight (MW)452.51
Molecular FormulaC23H28N6O4
CAS No.208848-19-5
Storage-20℃ for 3 years in powder formr
-80℃ for 2 years in solvent
SMILES CodeO=C(NC(C(C1=NN=C(C(C)(C)C)O1)=O)C(C)C)CN(C(C2=CC=CC=C2)=NC=C3N)C3=O
SynonymsONO-6818; ONO6818; ONO 6818; ONO-PO 736; Freselestat
ProtocolIn VitroSimulated extracorporeal circulation is established by recirculating fresh heparinized (3.75 U/mL) human blood for 120 minutes in a membrane oxygenator and a roller pump with and without 1.0 μM of Freselestat (ONO-6818). Neutrophil elastase levels are significantly lower in the Freselestat group. Freselestat significantly reduces interleukin 8 and C5b-9 production. Freselestat does not modulate changes of CD11b and L-selectin during recirculation.
In VivoFreselestat (ONO-6818; 10-100 mg/kg; oral administration; daily; for 8 weeks) treatment attenuates dose-dependently HNE-induced increases in lung myeloperoxidase activity, hemoglobin, and neutrophil count in bronchoalveolar lavage fluid. ONO-6818 inhibits acute lung injury induced by HNE by minimizing lung hemorrhage and accumulation of neutrophils in the lung.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM2.2099 mL11.0495 mL22.0990 mL44.1979 mL
5mM0.4420 mL2.2099 mL4.4198 mL8.8396 mL
10mM0.2210 mL1.1049 mL2.2099 mL4.4198 mL
20mM0.1105 mL0.5525 mL1.1049 mL2.2099 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
 
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Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
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Volume(start)
V1
 
=
 
Concentration(final)
C2
 
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Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O
Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.