FM-479

This product is for research use only, not for human use. We do not sell to patients.

FM-479
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
100mg$1500Check With Us
200mg$2250Check With Us
500mg$3795Check With Us

Cat #: V4533 CAS #: 2226521-64-6 Purity ≥ 99%

Description: FM 479 (FM479) is a negative control for FM-381 with no activity on JAK3 or other kinases.

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Molecular Weight (MW)442.51
Molecular FormulaC25H26N6O2
CAS No.2226521-64-6
SynonymsFM-479
ProtocolIn VitroFM-479 is the inactive control of FM-381, the concentration used in assays is similar.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM2.2598 mL11.2992 mL22.5984 mL45.1967 mL
5mM0.4520 mL2.2598 mL4.5197 mL9.0393 mL
10mM0.2260 mL1.1299 mL2.2598 mL4.5197 mL
20mM0.1130 mL0.5650 mL1.1299 mL2.2598 mL
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.