Diosmetin | CAS 520-34-3

Diosmetin

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
2g	$590	Check With Us
5g	$1150	Check With Us
10g	$1725	Check With Us

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Cat #: V0810 CAS #: 520-34-3 Purity ≥ 99%

Description: Diosmetin (also known as DIO; HSDB 8101; HSDB 8101; Luteolin 4'-methyl ether) is a naturally occuring flavonoid found in spearmint, oregano, and many other plants with various biological activity (e.g, antioxidant, anti-inflammation, anticancer).

References: Liu B, et al. Diosmetin induces apoptosis by upregulating p53 via the TGF-β signal pathway in HepG2 hepatoma cells. Mol Med Rep. 2016 Jul;14(1):159-64.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	300.26
Molecular Formula	C16H12O6
CAS No.	520-34-3
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 60 mg/mL (199.8 mM)r
	Water: <1 mg/mLr
	Ethanol: <1 mg/mL
SMILES Code	O=C1C=C(C2=CC=C(OC)C(O)=C2)OC3=CC(O)=CC(O)=C13
Synonyms	Luteolin 4-methyl ether; HSDB-8101; Diosmetin; HSDB 8101; HSDB 8101; Luteolin 4'-methyl ether

Protocol	In Vitro	Diosmetin inhibits cell proliferation in HepG2 cells in a concentration-dependent manner. Untreated HepG2 cells grow well and are observed to have with normal skeletons, whereas cells treated with diosmetin are distorted and a number of them become round and floating.
Protocol	In Vivo	Pretreatment with diosmetin significantly reduces serum levels of amylase and lipase; the histological injury; the secretion of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6; myeloperoxidase (MPO) activity, trypsinogen activation peptide (TAP) level, the expression of inducible nitric oxide synthase (iNOS); and the nuclear factor (NF)-κB activation in cerulein-induced acute pancreatitis.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.3304 mL	16.6522 mL	33.3045 mL	66.6089 mL
5mM	0.6661 mL	3.3304 mL	6.6609 mL	13.3218 mL
10mM	0.3330 mL	1.6652 mL	3.3304 mL	6.6609 mL
20mM	0.1665 mL	0.8326 mL	1.6652 mL	3.3304 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.