Decitabine (NSC-127716)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
1g	$250	In Stock
2g	$400	In Stock
5g	$675	In Stock

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Cat #: V0403 CAS #: 2353-33-5 Purity ≥ 98%

Description: Decitabine (also known as Deoxycytidine; 5-aza-dCyd; NSC 127716; 5-Aza-2'-deoxycytidine; deoxyazacytidine; dezocitidine; Brand name: Dacogen. Abbreviations: 5AZA; DAC) is a potent and orally bioactive inhibitor of DNA methylation (DNA methyltransferase) that is commonly used to treat myelodysplastic syndrome (MDS) and cute myeloid leukemia (AML).

References: Nakamura M, et al. Decitabine inhibits tumor cell proliferation and up-regulates E-cadherin expression in Epstein-Barr virus-associated gastric cancer. J Med Virol. 2016 Jul 19.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	228.21
Molecular Formula	C8H12N4O4
CAS No.	2353-33-5
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 45 mg/mL (197.2 mM)r
	Water: 10 mg/mL (43.8 mM)r
	Ethanol:<1 mg/mL
Solubility In Vivo	30% propylene glycol, 5% Tween 80, 65% D5W: 30 mg/mL
SMILES Code	O=C1N([C@H]2O[C@H](CO)[C@@H](O)C2)C=NC(N)=N1
Synonyms	5-Aza-2'-deoxycytidine; deoxyazacytidine; 5-aza-dCyd; Deoxycytidine; NSC127716; NSC 127716; NSC-127716; dezocitidine; Brand name: Dacogen. Abbreviations: 5AZA; DAC

Protocol	In Vitro	Decitabine treatment significantly inhibits cell growth of SNU719, NCC24 and KATOIII 96 hours after exposure to decitabine. Decitabine induces G2/M arrest and apoptosis in EBVaGC, inhibits invasion ability, and up-regulates E-cadherin expression for EBVaGC.
Protocol	In Vivo	Decitabine (1.0 mg/kg, p.o.) in combination with tetrahydrouridine (THU) causes severe toxicity occurs in female CD-1 mice, and results in an increased sensitivity to decitabine toxicity correlating with decitabine plasma levels.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	4.3819 mL	21.9096 mL	43.8193 mL	87.6386 mL
5mM	0.8764 mL	4.3819 mL	8.7639 mL	17.5277 mL
10mM	0.4382 mL	2.1910 mL	4.3819 mL	8.7639 mL
20mM	0.2191 mL	1.0955 mL	2.1910 mL	4.3819 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.