DAPI 2HCl | CAS 28718-90-3

DAPI 2HCl

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
250mg	$400	Check With Us
500mg	$650	Check With Us
1g	$975	Check With Us

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Cat #: V19170 CAS #: 28718-90-3 Purity ≥ 99%

Description: DAPI 2HCl, the dihydrochloride salt of DAPI, is a cell-permeable fluorescent probe and DNA-binding dye preferential to adenine and thymine rich DNA and can be used for flow cytometry.

References: Kapuscinski J, et al. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70(5):220-33.

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Molecular Weight (MW)	350.25
Molecular Formula	C16H17Cl2N5
CAS No.	28718-90-3

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.8551 mL	14.2755 mL	28.5510 mL	57.1021 mL
5mM	0.5710 mL	2.8551 mL	5.7102 mL	11.4204 mL
10mM	0.2855 mL	1.4276 mL	2.8551 mL	5.7102 mL
20mM	0.1428 mL	0.7138 mL	1.4276 mL	2.8551 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.