CLP257
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $850 | Check With Us |
| 200mg | $1275 | Check With Us |
| 500mg | $2150 | Check With Us |
: Cat #: V18558 CAS #: 1181081-71-9 Purity ≥ 99%
Description: CLP257 is a novel, potent and selective KCC2 K+-Cl- cotransporter activator (EC50 = 616 nM).
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| Molecular Weight (MW) | 307.34 |
|---|---|
| Molecular Formula | C14H14FN3O2S |
| CAS No. | 1181081-71-9 |
| SMILES Code | O=C1N=C(N2CCCCN2)S/C1=C\C3=CC=C(F)C=C3O |
| Synonyms | CLP 257; CLP-257; CLP257 |
| Protocol | In Vitro | There is no change in [Cl−]i in HEK293-cl cells when incubated with CLP257, indicating inactivity on NKCC1, KCC1, KCC3 or KCC4. Oocyte pre-incubation with CLP257 (200 nM) increases KCC2 transport activity by 61%, but causes no change in other CCCs. Functional, dose-dependent antagonism is also observed between CLP257 and the recently characterized KCC2 antagonist VU024055119. CLP257 (50 μM) provokes < 0.2% of the effect of 5 μM muscimol in CHO cells transduced with recombinant α1β2γ2 GABAA receptors, indicating negligible agonist activity of CLP257 on GABAA receptors. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.2537 mL | 16.2686 mL | 32.5373 mL | 65.0745 mL |
| 5mM | 0.6507 mL | 3.2537 mL | 6.5075 mL | 13.0149 mL |
| 10mM | 0.3254 mL | 1.6269 mL | 3.2537 mL | 6.5075 mL |
| 20mM | 0.1627 mL | 0.8134 mL | 1.6269 mL | 3.2537 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.