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CHELERYTHRINE CHLORIDE

This product is for research use only, not for human use. We do not sell to patients.

CHELERYTHRINE CHLORIDE
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Size Price Stock
500mg$1170Check With Us
1g$1850Check With Us
5g$4680Check With Us

Cat #: V3977 CAS #: 3895-92-9 Purity ≥ 98%

Description: Chelerythrine chloride (NSC-36405; NSC36405), a benzophenanthridine alkaloid extracted from the plant Greater celandine (Chelidonium majus), is a potent, cell-permeable, selective protein kinase C inhibitor with an IC50 of 660 nM.

References: Li W, et al. Effect of Chelerythrine Against Endotoxic Shock in Mice and Its Modulation of Inflammatory Mediators in Peritoneal Macrophages Through the Modulation of Mitogen-Activated Protein Kinase (MAPK) Pathway. Inflammation. 2012 Jul 24.

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Molecular Weight (MW)384.83
Molecular FormulaC21H18NO4.HCl
CAS No.3895-92-9
Storage-20℃ for 3 years in powder formr
-80℃ for 2 years in solvent
Solubility In VitroDMSO: 18 mg/mLr
Water: <1 mg/mLr
Ethanol: <1 mg/mL
SMILES CodeC[N+]1=CC2=C(OC)C(OC)=CC=C2C3=C1C4=CC5=C(OCO5)C=C4C=C3.[Cl-]
SynonymsNSC 36405; NSC36405; NSC-36405
ProtocolIn VitroChelerythrine inhibits the BclXL-Bak BH3 peptide binding with IC50 of 1.5 μM and displaces Bax, a BH3-containing protein, from BclXL. Mammalian cells treated with Chelerythrine undergoes apoptosis with characteristic features that suggest involvement of the mitochondrial pathway.
In VivoChelerythrine displays significant anti-inflammatory effects in experimentally induced mice endotoxic shock model in vivo through inhibition of LPS-induced tumor necrosis factor-alpha (TNF-α) level and nitric oxide (NO) production in serum.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM2.5986 mL12.9928 mL25.9855 mL51.9710 mL
5mM0.5197 mL2.5986 mL5.1971 mL10.3942 mL
10mM0.2599 mL1.2993 mL2.5986 mL5.1971 mL
20mM0.1299 mL0.6496 mL1.2993 mL2.5986 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.