Calciol (5,6-trans-Vitamin D3)

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
100mg	$3075	Check With Us
200mg	$4600	Check With Us
500mg	$7700	Check With Us

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Cat #: V40990 CAS #: 22350-41-0 Purity ≥ 99%

Description: Calciol (5,6-trans-Vitamin D3), the inactive form of vitamin D3, is an enantiomer of vitamin D3 with important biological roles in calcium homestasis. In vivo, 5,6-trans-vitamin D3 increases tibia ash weight and bone mineralization in vitamin D-deficient chicks. 5,6-trans-Vitamin D3 induces intestinal calcium transport and bone calcium mobilization in anephric rats fed a low-calcium and vitamin D-deficient diet. Calciol is a hydroxy seco-steroid that is (5Z,7E)-9,10-secocholesta-5,7,10(19)-triene in which the pro-S hydrogen at position 3 has been replaced by a hydroxy group. It is the inactive form of vitamin D3, being hydroxylated in the liver to calcidiol (25-hydroxyvitamin D3), which is then further hydroxylated in the kidney to give calcitriol (1,25-dihydroxyvitamin D3), the active hormone. It has a role as a human metabolite. It is a seco-cholestane, a hydroxy seco-steroid, a member of D3 vitamins, a secondary alcohol and a steroid hormone.

References: [1]. T C Chen, et al. An evaluation of the biologic activity and vitamin D receptor binding affinity of the photoisomers of vitamin D3 and previtamin D3. J Nutr Biochem. 2000 May;11(5):267-72.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	384.648
Molecular Formula	C27H44O
CAS No.	22350-41-0
SMILES Code	C=C1CC[C@@H](C/C1=CC=C2CCC[C@]3([C@]/2(CC[C@@]3([C@H](C)CCCC(C)C)[H])[H])C)O
Synonyms	Calciol; 5,6-trans-Cholecalciferol; Cholecalciferol EP Impurity A; 5,6-trans-Colecalciferol;

Protocol	In Vitro	Normal human keratinocytes in the cultured human keratinocytes by determining their influence on 3H-thymidine incorporation into DNA. 5,6-trans-vitamin D3 only induces significant inhibition at 10(-6) M. The dissociation constants of vitamin D receptor (VDR) for 5,6-trans-vitamin D3 is 560 nM.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.5998 mL	12.9989 mL	25.9978 mL	51.9956 mL
5mM	0.5200 mL	2.5998 mL	5.1996 mL	10.3991 mL
10mM	0.2600 mL	1.2999 mL	2.5998 mL	5.1996 mL
20mM	0.1300 mL	0.6499 mL	1.2999 mL	2.5998 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.