Calcifediol (25-hydroxy Vitamin D3)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
100mg	$1145	Check With Us
250mg	$1950	Check With Us
500mg	$2925	Check With Us

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Cat #: V2435 CAS #: 19356-17-3 Purity ≥ 98%

Description: Calcifediol (also known as 25-hydroxyvitamin D3; 25-hydroxy VD3) is the major circulating metabolite of vitamin D3 in the blood and is the form that is tested in medicine to determine vitamin D deficiency, namely, the test of 25-hydroxy vitamin D (calcifediol) levels to evaluate how much vitamin D is in the body.

References: Castoldi A, et al. Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections. Eur J Pharm Biopharm. 2016 Nov 22.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	400.64
Molecular Formula	C27H44O2
CAS No.	19356-17-3
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 80 mg/mL (199.7 mM)r
	Water: <1 mg/mLr
	Ethanol: 20 mg/mL (49.9 mM)
Solubility In Vivo	2% DMSO+30% PEG 300+5% Tween+ddH2O: 5mg/mL
SMILES Code	[H][C@]([C@]1(C)CCC/2)(CC[C@]1([H])[C@H](C)CCCC(C)(O)C)C2=C\C=C3C[C@@H](O)CCC\3=C
Synonyms	25-hydroxyvitamin D3;25-hydroxy VD3; 25-hydroxy Cholecalciferol; RO 8-8892; Rovimix Hy-D; U 32070E; 25-hydroxy Vitamin D3; 25-OH Vitamin D3; Calcifediol.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.4960 mL	12.4800 mL	24.9601 mL	49.9201 mL
5mM	0.4992 mL	2.4960 mL	4.9920 mL	9.9840 mL
10mM	0.2496 mL	1.2480 mL	2.4960 mL	4.9920 mL
20mM	0.1248 mL	0.6240 mL	1.2480 mL	2.4960 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.