BIX01294 triHCl
This product is for research use only, not for human use. We do not sell to patients.
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| Size | Price | Stock |
|---|---|---|
| 250mg | $920 | Check With Us |
| 500mg | $1450 | Check With Us |
| 1g | $2175 | Check With Us |
: Cat #: V3451 CAS #: 1392399-03-9 (HCl salt) Purity ≥ 98%
Description: BIX01294 triHCl (the tri-hydrochloride salt of BIX01294) is a novel and potent inhibitor of G9a histone methyltransferase with IC50 of 2.7 μM in a cell-free assay.
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| Molecular Weight (MW) | 600.02 |
|---|---|
| Molecular Formula | C28H38N6O2.3HCl |
| CAS No. | 1392399-03-9 (HCl salt) |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 98 mg/mL (163.3 mM)r |
| Water: >10 mg/mLr | |
| Ethanol: >10 mg/mL (13.33 mM) | |
| SMILES Code | CN1CCN(C2=NC(NC3CCN(CC4=CC=CC=C4)CC3)=C5C=C(OC)C(OC)=CC5=N2)CCC1.[H]Cl.[H]Cl.[H]Cl |
| Synonyms | BIX01294; BIX-01294; BIX 01294; N-(1-benzylpiperidin-4-yl)-6,7-dimethoxy-2-(4-methyl-1,4-diazepan-1-yl)quinazolin-4-amine trihydrochloride |
| Protocol | In Vitro | BIX-01294 trihydrochloride leads to necroptotic cell death in recurrent tumor cells. Necrostatin-1 (30 μM) partially reverses cell death induced by BIX-01294 (750 nM; 24 h) trihydrochloride. BIX-01294 (1 μM; 6 days) trihydrochloride causes the reduction in H3K9me2 levels in primary and recurrent tumor cells. BIX-01294 (1 μM) trihydrochloride significantly upregulates the canonical p53 targets Cdkn1a (p21) and Gadd45a in recurrent tumor cell lines. BIX-01294 (1 μM) trihydrochloride leads to a marked increase in phosphorylation of S345 of MLK. BIX-01294 (2 μM; 48 h) trihydrochloride selectively inhibits recurrent tumor cell growth. |
|---|---|---|
| In Vivo | BIX-01294 trihydrochloride (10 mg/kg; IP; three times a week for 2 weeks) significantly reduces tumor growth and tumor burden in recurrent tumor cells. Primary tumor growth is not inhibited. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 1.6666 mL | 8.3331 mL | 16.6661 mL | 33.3322 mL |
| 5mM | 0.3333 mL | 1.6666 mL | 3.3332 mL | 6.6664 mL |
| 10mM | 0.1667 mL | 0.8333 mL | 1.6666 mL | 3.3332 mL |
| 20mM | 0.0833 mL | 0.4167 mL | 0.8333 mL | 1.6666 mL |
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Step Two: Enter the in vivo formulation
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Method for preparing in vivo formulation:
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PEG300, mix and clarify, next add
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Tween 80,mix and clarify, next add
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ddH2O,mix and clarify.
Note:
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