Benserazide hydrochloride (Serazide; Ro 4-4602)

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
10g	$400	Check With Us
25g	$750	Check With Us
50g	$1125	Check With Us

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Cat #: V32716 CAS #: 14919-77-8 Purity ≥ 99%

Description: Benserazide HCl (Ro-44602; Serazide; Ro 4-4602), the hydrochloride salt of Benserazide, is a carbohydrazide-based, peripherally acting inhibitor of aromatic L-amino acid decarboxylase/AADC or DOPA decarboxylase and a medication used in combination with levodopa (madopar) to treat Parkinson's disease, parkinsonism, and restless leg syndrome.

References: Shen H, et al. Effects of benserazide on L-DOPA-derived extracellular dopamine levels and aromatic L-amino acid decarboxylase activity in the striatum of 6-hydroxydopamine-lesioned rats. Tohoku J Exp Med. 2003 Mar;199(3):149-59.

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Molecular Weight (MW)	293.70
Molecular Formula	C10H16ClN3O5
CAS No.	14919-77-8
Synonyms	Benserazide hydrochloride; Serazide; Ro 4-4602

Protocol	In Vitro	Benserazide hydrochloride (BH) and Levodopa (LD) individually and in combination (Benserazide hydrochloride + LD) (25 μM; 0 hour, 12 hours, 24 hours and 168 hours; SH-SY5Y) treatment inhibit protein aggregation and have the ability to minimise the amyloid-induced cytotoxicity in human neuroblastoma cell line. Benserazide hydrochloride and LD both can act as efficient inhibitors of the formation of cytotoxic HSA aggregates, and the inhibitory effects are more pronounced when both of these drugs are added simultaneously.
Protocol	In Vivo	Benserazide (5-50 mg/kg; intraperitoneal injection; male Wistar rats) treatment of 6-OHDA-lesioned rats increases exogenous L-DOPA-derived extracellular DA levels, the time to reach the peak DA levels are significantly prolong by Benserazide dose-dependently. The AADC activity in the denervates striatal tissues shows a significant decrease by 10 mg/kg and 50 mg/kg Benserazide. Benserazide reduces the central AADC activity in the striatum of rats with nigrostriatal denervation, which leads to changes in the metabolism of exogenous L-DOPA.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.4048 mL	17.0242 mL	34.0483 mL	68.0967 mL
5mM	0.6810 mL	3.4048 mL	6.8097 mL	13.6193 mL
10mM	0.3405 mL	1.7024 mL	3.4048 mL	6.8097 mL
20mM	0.1702 mL	0.8512 mL	1.7024 mL	3.4048 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.