BCECF-AM

This product is for research use only, not for human use. We do not sell to patients.

BCECF-AM
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
1mg$4603-6 Days
5mg$12503-6 Days

Cat #: V33702 CAS #: 117464-70-7 Purity ≥ 99%

Description: BCECF-AM, also called 2',7'-Bis- (2-Carboxyethyl)-5- (and-6)-Carboxyfluorescein, Acetoxymethyl Ester, is a novel and potent fluorescent indicator which is cell membrane permeable and is thus widely used as a fluorescent marker/indicator for measurement of intracellular pH. It is the Acetoxymethyl Ester form of BCECF.

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Molecular Weight (MW)820.70
Molecular FormulaC₄₀H₃₆O₁₉
CAS No.117464-70-7
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM1.2185 mL6.0924 mL12.1847 mL24.3694 mL
5mM0.2437 mL1.2185 mL2.4369 mL4.8739 mL
10mM0.1218 mL0.6092 mL1.2185 mL2.4369 mL
20mM0.0609 mL0.3046 mL0.6092 mL1.2185 mL
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.