Banoxantrone dihydrochloride (AQ4N)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
50mg	$450	To Be Confirmed
100mg	$750	To Be Confirmed
200mg	$1125	To Be Confirmed

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Cat #: V31201 CAS #: 252979-56-9 Purity ≥ 99%

Description: Banoxantrone 2HCl (AQ4N), the dihydrochloride salt of Banoxantrone, is a hypoxia-activated and bioreductive prodrug that can be reduced under hypoxia (e.g. in hypoxic cells) to a stable, compound AQ4, which is a potent topoisomerase II inhibitor with antitumor activity.

References: Hejmadi MV, et al. DNA damage following combination of radiation with the bioreductive drug AQ4N: possible selective toxicity to oxic and hypoxic tumour cells. Br J Cancer. 1996 Feb;73(4):499-505.

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Molecular Weight (MW)	517.40
Molecular Formula	C22H30Cl2N4O6
CAS No.	252979-56-9

Protocol	In Vitro	Banoxantrone (AQ4N) can be reduced in a hypoxic environment to a stable DNA-affinic agent AQ4. AQ4, a potent topoisomerase II inhibitor, would be capable of damaging cells recruited into the cell cycle following radiation damage to the well-oxygenated cells of the tumor
Protocol	In Vivo	Banoxantrone (AQ4N) can be reduced in a hypoxic environment to a stable DNA-affinic agent AQ4. AQ4, a potent topoisomerase II inhibitor, would be capable of damaging cells recruited into the cell cycle following radiation damage to the well-oxygenated cells of the tumor

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.9327 mL	9.6637 mL	19.3274 mL	38.6548 mL
5mM	0.3865 mL	1.9327 mL	3.8655 mL	7.7310 mL
10mM	0.1933 mL	0.9664 mL	1.9327 mL	3.8655 mL
20mM	0.0966 mL	0.4832 mL	0.9664 mL	1.9327 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.