Auristatin F

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
100mg	$850	To Be Confirmed
200mg	$1275	To Be Confirmed
500mg	$2150	To Be Confirmed

: [email protected] [email protected]

Cat #: V11901 CAS #: 163768-50-1 Purity ≥ 99%

Description: Auristatin F is an MMAF analogused as a cytotoxin in ADC (Antibody-drug conjugates) synthesis, acting as highly potent antimitotic agent/tubulin inhibitor and anticancer agent that inhibits tubulin polymerization.

References: [1]. Roy S, et al. SMI-Ribosome inactivating protein conjugates selectively inhibit tumor cell growth. Chem Commun (Camb). 2017 Apr 11;53(30):4234-4237.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	746.003
Molecular Formula	C40H67N5O8
CAS No.	163768-50-1
Storage	Powdert -20°C 3 yearsr
	4°C 2 yearsr
	*This product is not stable in solution, suggest to use it instantly.
Solubility In Vitro	DMSO : 200 mg/mL (268.10 mM; Under ultrasonic)
SMILES Code	CC[C@@H]([C@H](N(C([C@@H](NC([C@@H](N(C)C)C(C)C)=O)C(C)C)=O)C)[C@H](OC)CC(N1CCC[C@H]1[C@H](OC)[C@H](C(N[C@H](C(O)=O)CC2=CC=CC=C2)=O)C)=O)C
Synonyms	Auristatin F;

Protocol	In Vitro	Auristatin F and Monomethyl Auristatin F (MMAF) are potent ADC cytotoxin used in antibody-drug conjugates
	In Vivo	Auristatin F (5 mg/kg; i.v.; male Sprague-Dawley rats) has Cmax of 8276.76 ng/mL. The AUClast is 65661.30 min*ng/mL, and the clearance (CL) is 77.33 mL/min/kg, which is above the hepatic blood flow in the rat
	Animal model	Male Sprague-Dawley rats
	Dosages	5 mg/kg
	Administration	Intravenous injection

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.3405 mL	6.7024 mL	13.4048 mL	26.8095 mL
5mM	0.2681 mL	1.3405 mL	2.6810 mL	5.3619 mL
10mM	0.1340 mL	0.6702 mL	1.3405 mL	2.6810 mL
20mM	0.0670 mL	0.3351 mL	0.6702 mL	1.3405 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.