Anisodamine

This product is for research use only, not for human use. We do not sell to patients.

Anisodamine
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Size Price Stock
10g$450Check With Us
25g$800Check With Us
50g$1200Check With Us

Cat #: V10759 CAS #: 55869-99-3 Purity ≥ 99%

Description: Anisodamine is a ntural product extracted from Chinese solanacea plant, acting as a weak inhibitor/antagonist of alpha-1 adrenergic receptors and mAChRs.

References: Liu C, et al. Antishock effect of anisodamine involves a novel pathway for activating alpha7 nicotinic acetylcholine receptor. Crit Care Med. 2009;37(2):634-641.

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Molecular Weight (MW)305.37
Molecular FormulaC17H23NO4
CAS No.55869-99-3
ProtocolIn VitroAnisodamine (100 μg/mL; 20 minutes; RAW264.7 cells) pretreatment for 20 minutes before Ach results in significantly attenuates average fluorescence intensity of α-bungarotoxin binding compared with Ach alone
In VivoAnisodamine (50 mg/kg; i.p.; 72 hours) markedly decreases the mortality to 20%
Animal modelLPS-Induced Shock Mice
DosagesLPS-Induced Shock Mice
Administrationtion: I.p.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM3.2747 mL16.3736 mL32.7472 mL65.4943 mL
5mM0.6549 mL3.2747 mL6.5494 mL13.0989 mL
10mM0.3275 mL1.6374 mL3.2747 mL6.5494 mL
20mM0.1637 mL0.8187 mL1.6374 mL3.2747 mL
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.